SESRIC organised a five days training course on “The Use of Advanced Technology in Cotton Production” within the framework of the OIC Cotton Capacity Building Programme (OIC-CTP) in Lahore and Multan, Pakistan on 12-16 of December 2016.

The training course mainly focused on the following topics:

• Genetic modification of cotton
• Competent cells preparation.
• Agrobacterium inoculum preparation and electroporation
• Embryo isolation and treatment.
• Demonstration about biolistic gun
• Culturing of treated embryo on MS medium.
• Sub-culturing of developing embryo on selection medium
• DNA isolation from transgenic crops.
• Gel interpretation of isolated DNA
• Introduction to polymerase chain reaction, types and its use in GMO detection
• PCR for amplification of gene of interest
• Gel interpretation of amplified product
• mRNA expression of transgene through real time PCR
• Setting up of real time PCR for virus quantification
• Interpretation of results of real time PCR
• Development of Bt Cotton
• Role of biosafety studies in the commercialization of transgenic cotton
• Short hairpin RNA engineering and in planta gene silencing
• Quantification of Bt protein in transgenic cotton
• Hybrid seed production technology, transformation and expression studies of Cry1Ac+Cry2A and GT gene
• Fluorescent in situ hybridization of transgenic cotton for copy
• Expression analysis of cotton plants through microarray
• Cotton crop production and management
• Cotton varietal evaluation and testing system.

Photos